Standard PCR Reagents and Kits
Biotium PMA, 20mM in H2O
PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA. The PMA-modified dsDNA cannot be amplified by PCR. The dye is designed to be cell membrane-impermeable. Thus, in a population of live and dead cells, only dead cells are susceptible to DNA modification due to compromised cell membranes. This unique feature of PMA dye makes it highly useful in selective detection of live bacteria by qPCR. A bacterial sample can be first pre-treated with PMA under light to inactivate dsDNA from dead cells. DNA is then extracted and analyzed by PCR. PMA is also available in lyophilized format. Properties: Dark red liquid; Store at -20⁰C, protect from light; Ex/Em= 510/610 nm (following photolysis and covalent attachment to DNA/RNA); MW: 511.
Takara Bio EMERALDAMP MAX HS 160 RXN
PCR Master Mix, EmeraldAmp Max HS; 160 x 50uL rxns; Includes optimized buffer, high-yield hot start PCR enzyme, dNTP mixture, gel loading dye (green) and density reagent in 2X premix; High yield: 10X more end product
Crescent Chemical Co Inc DNTPS SET SOLUTION 100 MM
dNTPs Set, solution 100 mM molecular biology grade Set of 100 mM solutions of each dATP, dCTP, dGTP, dTTP.Free of DNase, RNase, Phosphatase and Protease. Absolutely free of substances that may inhibit PCR, e.g. pyrophosphates etc.Ultrapure dNTPs qualified for use in standar
BIOSEARCH TECHNOLOGIES INC (Lucigen) FailSafe PCR System, 1,000 U @2.5 U/LPCR System kit for end-point applications. Achieve consistent, high-fidelity results for every DNA template.
The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time.We promise!
LabScientific Aluminum Microtube Cooling Rack. Aluminum 200ul PCR Cooling Block (5 x 3 3/8”; 12.7 x 6 cm) 96 well (12 x 8 format)
Aluminum Microtube Cooling Rack. Aluminum 200ul PCR Cooling Block (5 x 3 3/8”; 12.7 x 6 cm) 96 well (12 x 8 format)
Thomas Scientific CHOICE TAQ BLUE MASTERMIX, 2MM MGC12, 400 REACTIONS
Choice Taq Blue Mastermix, 2Mm Mgc12, 400 Reactions
Bio Rad Laboratories ddPCR Supermix for Probes (No dUTP)
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200/QX100 Droplet Digital PCR Systems.
Meridian Life Science MyFi Mix, 500 x 50 uL reactions
Unique blend of highly-efficient MyTaq HS DNA Polymerase and a proprietary proofreading enzyme that combine to give increased target affinity for use with challenging templates and inhibitor-rich samples.
Biotium PMA Enhancer for Gram Negative Bacteria, 5X Solution
PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria. PMA Enhancer is provided as a 5X solution, and is added to a sample before the addition of dye. When a sequence from a gram-negative bacteria is amplified by PCR, samples pre-treated with Enhancer show a decrease in the signal from dead cells, with no change in the signal from live cells. Thus, PMA or PMAxx™ plus Enhancer is the optimal way to perform viability PCR on gram-negative bacteria. The mechanism by which Enhancer improves live/dead discrimination has not been determined. It may improve passive permeability of dead cell walls or membranes to the dye, and/or improve access of the dye to the dead cell DNA. The product comes with 16 ml of 5X PMA Enhancer for Gram Negative Bacteria solution.
Alpco Ltd GeneProof Varicella-Zoster Virus (VZV) PCR Kit
The GeneProof Varicella-Zoster Virus (VZV) PCR Kit provides superior sensitivity and specificity. For Research Use Only in North America. This kit is sufficient for 25 tests.
New England Biolabs, Inc. LongAmp Taq PCR Kit – 100 reactions (50 µl vol)
The LongAmp Taq PCR Kit features LongAmp Taq DNA Polymerase, which is a unique blend of Taq and Deep Vent DNA Polymerases. The 35 exonuclease activity of Deep Vent Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq Polymerase alone. The LongAmp Taq PCR Kit is supplied with LongAmp Taq Reaction Buffer, which is optimized to give highest PCR yields up to 30 kb. The kit can generate a wide range of PCR products, up to 30 kb from plasmid or from human genomic DNA.